Brassica napus, germinlike proteins glps, oxidative burst, plant disease resistance, sclerotinia sclerotiorum. Frontiers emerging trends in molecular interactions between. In the present study, twentyone germin gene members in soybean cultivar. In the present study, we report genomewide identification, characterisation, subcellular localization, enzyme activity, and expression analysis of the glp gene family. Bernsdorff and daguang cai, members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum, journal of experimental botany, 63, 15, 5507, 2012. A protein blast search of rsppd against the arabidopsis proteome uncovered high homology to members of the mes family of arabidopsis vlot et al. Transcriptomic comparison between brassica oleracea and rice. Germinlike proteins glps are ubiquitous watersoluble glycoproteins that are located in the extracellular matrix. Segmental structure of the brassica napus genome based on comparative analysis with arabidopsis thaliana isobel a. Transient expression of members of the germinlike gene family in epidermal cells of wheat confers disease resistance. Major haplotype divergence including multiple germinlike protein genes, at the wheat sr2 adult plant stem rust resistance locus. A total of 21,237 sage tags were sequenced and 4,211 unique tags were identified. The name germin was given because the first described member of the family, wheat germin, was discovered in germinating wheat grains. Germin like proteins glps are watersoluble plant glycoproteins belonging to the cupin superfamily.
Sclerotinia minor jagger is the causal agent of sclerotinia blight, a highly destructive disease of peanut arachis hypogaea. Members of the germinlike protein family in brassica napus. To explore mapkkk functions in biotic and abiotic stress. Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of. Members of the germinlike protein family in brassica napus are candidates.
May 26, 2016 rietz s, bernsdorff fe, cai d 2012 members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum. It has been suggested that disease symptoms are associated with clogging of xylem vessels. The brad gff3 varies so widely in its attribute formatting from most gff3 files that ive hardly been able to get it to work with rnaseq related software. Factors affecting pathogenicity of the turfgrass dollar spot. Germins and germinlike proteins glps constitute a large and highly diverse family of ubiquitous plant proteins. Dec 18, 2008 verticillium longisporum is one of the most important pathogens of brassicaceae that remains strictly in the xylem during most stages of its development. Pdf identification and analysis of the germinlike gene family in. The basic helixloophelix bhlh is the second largest gene family in the plant, some members play important roles in pistil development and response to drought, waterlogging, cold stress and salt stress. Relationship between disease resistance and rice oxalate.
This work demonstrates that a brassica mirna, which is subject to dynamic regulation during development and responds to viral infection, regulates immunity and development through distinct modes of action. The brassica genus is a member of brassicaceae cruciferae and contains 39 species. Previous studies have shown that germinlike proteins glps are present ubiquitously in rice and arabidopsis. Phylogenetic tree of members of the mir169 family in brassica napus and arabidopsis thaliana based on their precursor sequences using the neighbor. Members of the germinlike protein family in brassica. Background germinlike superfamily members are ubiquitously. Free fulltext pdf articles from hundreds of disciplines, all in one place identification and functional analysis of mitogenactivated protein kinase kinase kinase mapkkk genes in canola brassica napus l. Research article open access genomewide identification. Comparative analysis of basic helixloophelix gene family. During infection, large, whitegrey lesions form on the stems of the host plant, perturbing seed development and decreasing yield.
Pdf functional characterization of germin and germinlike protein. The tradeoff between growth and defense has long been a major challenge in crop breeding. Cloning and expression analysis of zmglp1, a new germinlike. Molecular cloning and characterization of novel morus alba.
In this study, proteins from xylem sap of adult brassica napus plants were precipitated by acetone, separated by 2de and partial amino acid sequences were determined by tandem mass spectrometry. As demonstrated before, the xylem sap collected with the employed method showed no detectable contaminations from phloem sap or other. Harmon2,6, wei sha1,3 and sixue chen2,4,6 1 college of life. They are part of the cupin superfamily of biochemically diverse proteins, a superfamily that has a conserved tertiary structure, though with limited similarity in primary sequence. Genomic organization and structural diversity of germinlike. Brassica napus xylem sap contains only low concentrations of amino acids, and aromatic amino acids are especially scarce singh et al. Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis. The brassica napus receptorlike protein rlm2 is encoded by a second. The low affinity sulfate transporter last 3 family protein encoded by genes in a gene cluster was identified as the target of two distinct mirnas ptomir395a and ptomir395bk. The brassica napus bkr enzyme is nadphdependent and forms part of a dissociable type ii fatty acid synthetase fas.
Chromosome geographical name count distribution habitat b. The brassica genus consists of approximately 100 species, including species brassica napus l. Functional characterization of germin and germinlike. A role of some family members in defense against pathogen attack. This study describes the identification of a family of 14 germinlike genes from brassica napus bnglp designated bnglp1bnglp14 and investigated potential functions of bnglps in plant defense. Analysis of xylem sap proteins from brassica napus bmc. Proteomic approach to understand the molecular physiology. Germin and germinlike proteins glps are encoded by a family of genes found in all plants. Arabidopsis thaliana contains a large family of germinlike proteins. Worldwide, canola is the third most important edible. Because of extensive sequence information from genome or expressed sequence tag est sequencing programs, it became clear that germin and glps belong to a multigene family in plants, including arabidopsis arabidopsis thaliana, rice oryza sativa, and barley subsp. Rietz s, bernsdorff fe, cai d 2012 members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum.
Department of molecular phytopathology, institute of phytopathology, christianalbrechtsuniversitat of kiel, germany, hermann, rodewald str. Here, a novel glp gene was isolated from gossypium hirsutum and designated as ghabp19. First, identification and characterization of additional members of the burp gene family in soybean could be of practical importance. Thereby, rsppd clustered with the members of subfamily 2, as determined by yang et al. Integrated mrna, srna, and degradome sequencing reveal. In this study, we identified a total of 34 fulllength aap genes representing eight subgroups aap18 from the allotetraploid rapeseed genome a na nc nc n,2n4x38.
Brassica napus, bor transporter, b deficiency, transcriptional profile, b efficiency, gene function background boron b is an essential micronutrient for higher plants 1. In the present study, the characterization of the spatial, developmental, drought, and abainduced expression of three nsltp gene family members are presented. Microbial relatives of the seed storage proteins of higher. Brassica as a crop and raphanus, sinapis, and brassica as weeds.
Overexpression of germinlike protein gmglp10 enhances. Pdf germin and germinlike proteins constitute a ubiquitous family of plant proteins. The colocalisation of the blackleg resistance genes lepr3 and rlm2 in the b. However, little is known about cotton glps in relation to fungal pathogens. Pdf characterization of peanut germinlike proteins. Expression of an oxalate oxidase gene in tomato and. Identification and analysis of the germinlike gene family in. Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum article fulltext available. Identification and analysis of mkk and mpk gene families in. Taro colocasia esculenta transformed with a wheat oxalate. In the available sequence databases, we identified a germinlike gene family in b.
Bootstrap confidence values were obtained using replicates and only more than 75% of replications are shown. Jun, 2015 rietz s, bernsdorff fem, cai d 2012 members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum. Verticillium longisporum, the invisible threat to oilseed. Members of the germinlike protein family in brassica napus are. Germinlike proteins glps are watersoluble plant glycoproteins belonging. Interestingly, the number of unique tags in pollen was low compared with the sage library of the leaf constructed on a similar scale.
Based on evidence that oxalic acid is involved in the pathogenicity of many sclerotinia species, our objectives were to recover transgenic peanut plants expressing an oxalic aciddegrading oxalate oxidase and to evaluate them for increased resistance to s. These proteins have been reported to play vital roles in diverse biological processes. The bhlh gene family has been identified in many species, except for brassica oleracea and b. Brassica napus glp genes are induced as early as 6 hpi with s. The protein index takes a little more than 2 gigabytes.
The adult plant stem rust resistance gene sr2 was introgressed into hexaploid wheat cultivar cv marquis from tetraploid emmer wheat cv yaroslav, to generate stem rust resistant cv hope in the 1920s. Significant sequence similarity is observed with enoyl acyl carrier protein reductase enr, the other reductase of fas, and the shortchain alcohol dehydrogenase sdr family. Taro is a tropical root crop that is grown widely throughout the pacific, africa, asia, the west indies, and south america plucknett et al. In australia, other important cropping weeds from the brassicaceae family include hirschfeldia incana, diplotaxis spp. Research article open access genomewide identification of. Colocalisation of the blackleg resistance genes rlm2 and. An increased production of plant secondary metabolites in response to v. Characterization of peanut germinlike proteins, ahglps in. In the present study, twentyone germin gene members in. Xylem protein extraction, separation and identification. Genomewide identification of brassica napus micrornas and.
However, few systematic analyses of aaps have been reported in brassica napus so far. Rietz s, bernsdorff fem, cai d 2012 members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum. It was the fifth most harvested root crop in the world with production estimated at 9. We have characterized the global gene expression patterns of arabidopsis pollen using serial analysis of gene expression sage. Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum steffen rietz, friederike e. Ive generated genome indices via star using two brassica napus reference genome sources.
The nectaryspecific pattern of expression of the tobacco nectarin i promoter is regulated by multiple promoter elements. Mar 31, 2016 members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum. Origins and diversity of brassica and its relatives 5 table 1. In the present study, twentyone germin gene members in soybean. A brassica mirna regulates plant growth and immunity. Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia. Jul 31, 2014 phylogenetic tree of members of the mir169 family in brassica napus and arabidopsis thaliana based on their precursor sequences using the neighbor. Identification, structure, and differential expression of. Nucleotide sequence of a member of the napin storage protein family from brassica napus received for publication, december 19, 1986. Pdf the important role of germins ger and genes coding for germinlike proteins glp in responses against. Tmtbased quantitative proteomics analyses reveal novel. Jun 30, 2016 read tmtbased quantitative proteomics analyses reveal novel defense mechanisms of brassica napus against the devastating necrotrophic pathogen sclerotinia sclerotiorum, journal of proteomics on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. This study aims to identify the bhlh family members in b. Use of serial analysis of gene expression technology to.
To identify bnglp members with a putative function in defence against s. Caimembers of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum. Brassica napus is one of the most importantly economical and biofuel crops. Mes16, a member of the methylesterase protein family. The aim of our study was to investigate extracellular defence reactions induced by v. Identification and analysis of the germinlike gene family in soybean.
Oct 01, 2015 read sunflower germinlike protein haglp1 promotes ros accumulation and enhances protection against fungal pathogens in transgenic arabidopsis thaliana, plant cell reports on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Like most of jims software interactive use on this web server is. Genomewide identification and characterization of nodule. Pei y, li x, zhu y, ge x, sun y, liu n, jia y, li f and hou y 2019 ghabp19, a novel germinlike protein from gossypium hirsutum, plays an important role in the regulation of resistance to verticillium and fusarium wilt pathogens. Read sunflower germinlike protein haglp1 promotes ros accumulation and enhances protection against fungal pathogens in transgenic arabidopsis thaliana, plant cell reports on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum. Ijms free fulltext genomewide characterization and. The index is used to find areas of probable homology, which are then loaded into memory for a detailed alignment. Proteins of the plasma membrane related with the ecs. Pdf members of the germinlike protein family in brassica. Protein blat works in a similar manner, except with 4mers rather than 11mers. Bootstrap confidence values were obtained using replicates and only more than 75% of. Two members of the thioredoxinh family lnteract with the. Ijms free fulltext transcriptomewide identification of.
A role of some family members in defense against pathogen attack had been proposed based on gene regulation studies and transgenic approaches. Three droughtresponsive members of the nonspecific lipid. Characterization of peanut germinlike proteins, ahglps in plant. Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum regulation of two germinlike protein genes during plum fruit development regulation of two germinlike protein genes during plum fruit development. Ghabp19, a novel germinlike protein from gossypium. Sclerotinia stem rot ssr caused by the phytopathogenic fungus sclerotinia sclerotiorum is a major disease of oilseed rape brassica napus. Identification and functional analysis of mitogenactivated. The multigene family encoding germinlike proteins of. Bernsdorff and daguang cai, members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum, journal of experimental botany, 10. Identification and analysis of the germinlike gene family.
In the present study, a glp in soybean glycine max l. The mapkkks comprise the largest family in the mapk cascades, yet only a few of these genes have been associated with physiological functions, even in the model plant arabidopsis thaliana. Members of the germinlike protein family in brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of sclerotinia sclerotiorum pp. Regulation of two germinlike protein genes during plum fruit development regulation of two germinlike protein genes during plum fruit development. Identification and characterization of micrornas in oilseed. Genomewide characterization and expression analysis of the. The fungal pathogen sclerotinia sclerotiorum infects a broad range of dicotyledonous plant species and causes stem rot in brassica napus. Identification and characterization of micrornas in. It is therefore possible that different gene family members account for the observed diversity in patterns of expression, each one perhaps performing a different function. Brassica napus, germinlike proteins glps, oxidative burst, plant disease resistance, sclerotinia sclerotiorum, superoxide dismutase. This document provides an overview of baseline biological information relevant to risk assessment of genetically modified forms of the species that may be released into the australian environment.
Frontiers ghabp19, a novel germinlike protein from. Enhancing resistance to sclerotinia minor in peanut by. However, the understanding regarding their role in development and abioticbiotic stress resistance remains limited. The genus name comes from the classical latin word brassica, referring to cabbage and other cruciferous vegetables. Using the yeast two hybrid system, we isolated two members of the thioredoxinh family that interact specifically with the srk910 protein kinase domain.
We chose to work with the burp family for a variety of reasons. Examples of the diversity and characteristics of selected natural brassica species. Segmental structure of the brassica napus genome based on. Germin and germinlike proteins constitute a ubiquitous family of plant proteins.
Cai d 2012 members of the germinlike protein family in brassica napus are. The control of sclerotinia stem rot on oilseed rape brassica. The important role of glps in plant responses against various abiotic and biotic stresses, especially pathogens, is well validated. Comparative analysis of cdresponsive maize and rice.
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